Figure 4.

(A) Effect of minicells_azurin on the migration of CT26/HT29 colon cancer cells. (B) Mouse CT26 colon cancer cells and human HT29 colon cancer cells migration toward a chemoattractant (culture media supplemented with FBS) was evaluated in Transwell assays. Cells were treated with CM from minicells, minicells_azurin, and cancer cell media (culture media control) for 24 h, and cell migration was quantified. Results are presented as the percentage of invasive cells compared to the control condition (p-values compare % of cancer cell invasiveness between minicells_azurin CM treatment and the remaining treatment conditions; n = 3). (C) Effect of minicells_azurin on the invasion of CT26/HT29 colon cancer cells. (D) Human HT29 colon cancer cells and mouse CT26 colon cancer cells invasion toward a chemoattractant (culture media supplemented with FBS) was evaluated in Matrigel invasion assays. Cells were treated with CM from minicells, minicells_azurin, and cancer cell media (culture media control) for 24 h, and cell migration was quantified. Results are presented as the percentage of invasive cells compared to the control condition (p-values compare % of cancer cell invasiveness between minicells_azurin’ CM treatment and the remaining treatment conditions; n = 3). The significance was determined by a ONE-WAY ANOVA (*** p < 0.001, ** p < 0.01, * p < 0.05, ns: no significance).