Figure 14.

The characterization of exosomes isolated from bovine and human milk via transmission electron microscopy (TEM). (A,B) Typical electron microscopy equipment and setup used for exosome analysis. In our laboratory, we routinely use the Hitachi HT7800 model. (C–F) Representative TEM images of isolated exosomes negatively stained with 0.5% uranyl acetate are depicted. Size markers are depicted in each images that were taken at original magnifications of (C) 10,000×, (D) 20,000×, and (E,F) 25,000×, respectively.