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. 2023 Sep 16;17(20):19613–19624. doi: 10.1021/acsnano.3c01534

Figure 2.

Figure 2

ELV cargo tunability, uptake, and induction of pro-angiogenic response when administered to CECs. (A) Tunability of miR-126 loading into ELVs by modulating the number of electroporation pulses in a square-wave electroporating setup. Red highlight shows maximum miR loading (eight pulses). (B) Uptake of calcein + sEVs and electroporated ELVs by 2D culture of CECs. (C) Calcein-AM + CECs (green) treated with electroporated miR-126+ ELVs or sEVs incubated on Matrigel form tubes overnight. (D) Quantification of angiogenic parameters of total tube length and total segment length show an increase after ELVs compared to sEV treatment. Data normalized to a negative control. Mean ± SEM. Significance was tested with one-way ANOVA with a Tukey posthoc and two-way Student’s paired t test. n.s. = not significant. *P < 0.05.