Figure 7.

Effect of added H₂0₂ on TLR4/NF-κB inflammation pathway. Schematic diagram for LPS-induced activation of TLR4/NF-κB inflammation pathway and effect of NIR light (A) or 100 μM H₂O₂ (B) treatment in HEK-Blue^TM hTLR4 cells. HEK-Blue^TM hTLR4 cells were LPS-induced for the inflammatory response and maintained in an inflamed state via two additional LPS boosts (blue arrows) and subjected to (A) brief 10-min intervals of NIR light exposure or (B) 100 μM H₂O₂ treatment. Both treatments (red arrows) were repeated once every 12 h, over a 48-h trial period. ((C,D)) Measurement of inflammation via colorimetric assay in control without LPS and LPS-induced HEK-Blue^TM hTLR4 not treated (black bars) or treated (red bars) with NIR light (C) or H₂O₂ (D). n = 5, * p < 0.1; ** p < 0.01; *** p < 0.001.