Figure 1.

(a) Western blotting (WB) for the ALIX protein displayed an increased expression of this protein in isolated vesicles compared with that in isolated cells (cell line E-20); (b) CD63 immunoblot demonstrated strong expression of this protein in isolated vesicles compared with that in isolated cells (E-20 cell line); (c) calnexin expression was tested with immunoblotting and was detected only in cell isolates, confirming the purity of the isolated vesicles; (d) GRP78, another specific cellular protein, was also detected only in the isolated cells, further validating the purity of the isolated vesicles.