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Cytoplasmic labeling in the E-20 cell line. The decrease in cells was more evident when treated with EVs and melatonin for 24 hat a concentration of 1 mM (scale bar: 50 μm). Each column corresponds to the mean + standard error of triplicates. Significant values (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001) and nonsignificant values (ns) were determined using ANOVA followed by Tukey’s test.
