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. 2023 Sep 24;13(10):1437. doi: 10.3390/biom13101437

Figure 2.

Figure 2

Effects of IL-17 and Dox on PDLSCs growth and migration: (A) Proliferation of PDLSCs determined by MTT test after 24, 48 and 72 h of incubation with IL-17 (50 and 100 ng/mL) and Dox (100 ng/mL). Absorbance values were normalized to the control level and presented as the mean ± SEM from three independent experiments. (B) Expression of proliferation marker Ki67 in PDLSCs. Cells were labeled with Ki-67 primary antibody and corresponding secondary FITC-conjugated antibody along with DNA stain DAPI. Representative images of immunofluorescence microscopy obtained in three independent experiments are shown. Scale bars: 20 μm. Graphical presentation of ki67 protein expression as analyzed by Image J, relative protein expression normalized to control. Results in graphs are presented as mean ± SEM from at least three independent experiments (n = 3). Statistically significant differences: ** p < 0.01 compared to control. (C) Migration of PDLSCs analyzed by scratch assay: after a scratch was made in the confluent monolayer, cells were incubated in GM with 0, 50 and 100 ng/mL IL-17 and Dox 100 ng/mL for 24 h. Representative pictures from four experiments (each performed in triplicate) are presented. The graph represents the percentage of the scratch area covered with migrating cells compared to corresponding nontreated control (100%). Results in graphs are presented as mean ± SEM from at least three independent experiments. Significant difference versus corresponding control indicated by darts and calculated by t-test: ** p < 0.01, *** p < 0.001, ### p < 0.001. (D) PDLSCs were immunostained with phalloidin and (E) vimentin primary antibody, corresponding TRITC-conjugated secondary antibody and DAPI. Representative images of three independent experiments are shown. Scale bars: 10 μm. Graphical presentation of phalloidin and vimentin protein expression as analyzed by Image J, relative protein expression normalized to control. Results in graphs are presented as mean ± SEM from at least three independent experiments. Statistically significant differences: ** p < 0.001, *** p < 0.001 compared to control, # p < 0.05 ### p < 0.001 compared to Dox.