Table 2.
Sequences of primers used for qRT-PCR analysis.
| Genes | Sequence (5′-3′) | Amplicon Size (bp) |
Amplification Efficiency | Accession No. |
|---|---|---|---|---|
| Beta-actin (β-actin) | F: TGCGTGACATCAAGGAGAAGC | 176 | 1.92 | XM_044169301.1 |
| R: GAGGAAGGAAGGCTGGAAGAG | ||||
| Ribosomal protein L13a (rpl13a) | F: CACCCTATGACAAGAGGAAGC | 100 | 2.01 | MK770673 |
| R: TGTGCCAGACGCCCAAG | ||||
| Nuclear factor erythroid 2-related factor (nrf2) | F: ACGAAAGCGAAAGCTCCTCA | 90 | 1.89 | MT270449.1 |
| R: GCTCTCTTCCAGAATGGCGT | ||||
| Kelch-like ECH-associated protein 1 (keap1) | F: GTGGCAACCCAGGAGGAG | 187 | 1.82 | XM_044189604.1 |
| R: GGGAATGGCAACGGACA | ||||
| Glutathione reductase (gr) | F: CAGGCATCCTTTCCACCC | 178 | 2.11 | XM_044204922.1 |
| R: TCCAGTCCTCTGTCCGTTTTA | ||||
| Superoxide dismutase (sod) | F: CACGCTCCCTGACCTGACA | 176 | 1.83 | XM_044168059.1 |
| R: GGAGGGCAACCTGTGCTG | ||||
| Catalase (cat) | F: GCGTTTGGCTACTTTGAGGT | 108 | 1.82 | XM_044194118.1 |
| R: CACAGTGGAGAAGCGGACA | ||||
| Glutathione peroxidase (gpx) | F: GCCCATCCCCTGTTTGTG | 185 | 1.92 | XM_044172415.1 |
| R: AACTTCCTGCTGTAACGCTTG | ||||
| GRP78 immunoglobulin heavy chain-binding protein (bip) | F: GGCCACTAAGGATGCTGGAA | 136 | 1.84 | XM_044173053.1 |
| R: ACCACCCAAATCGAACACGA | ||||
| Inositol-requiring protein-1 (ire1) | F: CATACAGGTCAGTTTCTGCTACAC | 106 | 1.81 | XM_044184760.1 |
| R: AAATCAACATCCCTGCCACCT | ||||
| Eukaryotic translation initiation factor 2-alpha kinase 3 (perk) | F: TGCTGGAGTCATCCTACCGA | 113 | 1.83 | XM_044166231.1 |
| R: CGCAGAGCAGATGTACCGAA | ||||
| Activating transcription factor 6 (atf6) | F: AGATGAGTTGCTTGAGGCCC | 150 | 1.86 | XM_044198830.1 |
| R: GCAGGTGACAGAGAGTCCAC | ||||
| X-box-binding protein 1 (xbp1) | F: AAACAGGGTGCTTCGGGAAA | 125 | 1.9 | XM_044196969.1 |
| R: CATTCCCGGTGGACAACAGA | ||||
| Eukaryotic translation initiation factor 2A (eif2a) | F: CTGTGCACACCCCCTTATGT | 241 | 1.79 | XM_044178962.1 |
| R: CGTGGATGGACGGGTAATGT | ||||
| Activating transcription factor 4 (atf4) | F: TGCACTGGCTATTTCTGGCAA | 131 | 1.92 | XM_044187002.1 |
| R: ATTTGGTCATGCTTTGGCCG | ||||
| DNA-damage-inducible transcript 3 (chop) | F: AACGGTGCCTTGTCCACTTT | 122 | 1.77 | XM_044216843.1 |
| R: TCCGTCAGCTCCTGTACCTT | ||||
| Caspase 9 (casp9) | F: ACACAGGCTTTGAGGTGTCC | 157 | 1.81 | XM_044210980.1 |
| R: AGAATGTCACATGGGGTGGG | ||||
| Caspase 3 (casp3) | F: ACAGGTGCTACGCCTCATTC | 172 | 1.82 | GU178032 |
| R: CCTCTGCAAGCCTGGATGAA | ||||
| B-cell lymphoma-2 (bcl2) | F: CCAGAAAACATTCCACCAAAG | 148 | 1.8 | XM_044197230.1 |
| R: GGGAGATGAGTAAGGAAGGGA | ||||
| Bcl2-associated X protein (bax) | F: TCCTACTTTGGCACACCCAC | 108 | 2.07 | XM_044180218.1 |
| R: TGTCTGCTCTTCACGAACCC | ||||
| Unc-51 like kinase 1 (ulk1) | F: GTGCCTGCCCAGTTTCCC | 268 | 1.76 | XM_044195569.1 |
| R: GCAGGTTCTGTTCCATACGCT | ||||
| Beclin 1 (becn1) | F: AGGAGGTGAAGAGCGATAAGG | 123 | 2.06 | XM_044167873.1 |
| R: CCAGGCGACGGTTGTGA | ||||
| Microtubule-associated protein 1 light chain 3b (lc3b) | F: AGAGCAGCACCCCAGCAA | 182 | 1.9 | XM_044194970.1 |
| R: CGTTGACCAGCAGGAAGAAA | ||||
| Autophagy related 4c cysteine peptidase (atg4c) | F: TCAGCACCAGCGATTTCCC | 181 | 2.15 | XM_044200084.1 |
| R: GCGGGGTATTTCTCCTTCG |