Fig. 4.

Validation of cascade circuit libraries at the transcriptional level. a Large time interval circuit. b Small time interval circuit. c Intermediate time interval circuit. d Circuit with no time interval. The strains were continuously cultured in a constant temperature microplate shaker for 12 h. Samples were taken at 1 h, 2 h, 3 h, 4 h, 6 h, 9 h, and 12 h and then subjected to real-time fluorescence quantitative PCR