Skip to main content
. 2023 Sep 28;12(10):1809. doi: 10.3390/antiox12101809

Figure 3.

Figure 3

Effect of L. bicolor honey extract on cell viability, NO production in RAW 264.7 cells, and the mRNA expression of key inflammation- and oxidation-related cytokine genes in LPS-stimulated RAW 264.7 cells. (A) Effects of L. bicolor honey extract on the viability of RAW 264.7 cells. RAW 264.7 cells were treated with the indicated concentrations of L. bicolor honey extract for 24 h. # p ˂ 0.05 compared to the blank group. (B) Effects of L. bicolor honey extract on LPS-induced NO production in RAW 264.7 cells. Cells were pretreated with/without the indicated concentrations of L. bicolor honey extract for 1 h and then stimulated with LPS (1 µg/mL) for 24 h. LPS group referring to cells were treated with LPS in the absence of L. bicolor honey extract. The blank group referring to cells was treated with neither LPS nor L. bicolor honey extract. The relative transcript levels of (C) IL-10, (D) COX-2, (E) iNOS, (F) TNF-α, and (G) HO-1 were quantified using qRT-PCR. Cells were pretreated with L. bicolor honey extract at the indicated concentrations for 1 h and then stimulated with LPS (1 µg/mL) for 6 h. ### p ˂ 0.001, compared to the blank group, which was not treated with L. bicolor honey extract or LPS; * p ˂ 0.05, **p ˂ 0.01, and ***p ˂ 0.001, compared to LPS group.