Determining the antimicrobial activity of modified CLEC3A-derived peptides RK-9 and WRK-9. (a) Antimicrobial activity assay using LL-37 (* p = 0.028), HT-16, HT-47, ST-16, DK-29, RK-9 (** p = 0.005), and WRK-9 in P. aeruginosa. The bar chart shows the determined MIC50 of the peptides. (b) Antimicrobial activity assay using LL-37, HT-16, HT-47, ST-16, DK-29, RK-9, and WRK-9 in S. aureus. The bar chart shows the determined MIC50 of the peptides (c) Antimicrobial activity assay using LL-37, HT-16, HT-47, ST-16, DK-29, RK-9, and WRK-9 in MRSA. The bar chart shows the determined MIC50 of the peptides including the numerical values with confidence intervals as the legend. Depicted are averages and standard deviations. Statistical significance was calculated using Prism 9 with a paired ANOVA test followed by a Dunett test and multiple comparisons, comparing each peptide with HT-16. All experiments were repeated three times (n = 3). na: not applicable.