Skip to main content
. 2023 Oct 11;12(10):1532. doi: 10.3390/antibiotics12101532

Figure 7.

Figure 7

Cytotoxicity, CD spectroscopy, and biostability of modified CLEC3A-derived peptide dWRK-30. (a) Cytotoxicity assay of NIH3T3 cells with LL-37 (** p = 0.0038), DK-29, WRK-30, and dWRK-30 (* p = 0.0354), Vancomycin, Colistin (* p = 0.0219), Untreated, and TritonX (** p = 0.0015). All values are percentages normed to the untreated controls (untreated controls were set as 100%). Depicted are averages and standard deviations. Statistical significance was calculated using Prism 9 with a paired ANOVA test followed by a Dunett test and multiple comparisons, comparing each treatment with the untreated control. All experiments were repeated three times (n = 3). (b) CD spectroscopy of dWRK-30 (black) and WRK-30 (dark grey) in phosphate buffer (PB). (c) CD spectroscopy of dWRK-30 (black) and WRK-30 (dark grey) in 50% TFE in PB. All CD spectroscopy experiments were performed three times (n = 3). (d) Representative SDS-PAGE of dWRK-30 samples incubated in murine serum (S) The image of the SDS-PAGE is cropped in width and length. Full-sized SDS-PAGE gels are shown in Supplementary Figure S8. SDS-PAGE experiments were performed three times (n = 3).