Figure 4.

Morphological changes in two non-malignant, HEK-293 (top) and MRHF (middle), cell lines and one malignant, HT-29 (bottom), cell line induced by differential treatments after 24 h. Cells were either left untreated or treated with 1% DMSO (vehicle control), 100 µM CDDP (positive apoptotic control) or treated with 10 µM complex 1 and 2, respectively. Typical morphological changes to cells diagnostic of apoptosis, such as cell shrinkage, rounding and apoptotic blebbing and bodies, are indicated by the red arrows. Light micrographs were taken with a Zeiss Axiovert 25 inverted light microscope using Axio Vision 3.1 software at 200× magnification.