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. 2023 Oct 14;11(10):2794. doi: 10.3390/biomedicines11102794

Figure 6.

Figure 6

Mitochondrial potential and cellular plasma permeabilization were quantified in malignant HT-29 cells using a Muse® MitoPotential kit. Cells were either left untreated or treated with 1% DMSO, 200 µM CDDP or 10 µM and 20 µM of selected complexes 1 and 2 for 24 h. Averaged percentages were calculated and are shown within the dot plots followed by the ±SEM (n = 3). Asterisks indicate significant differences between treatments and 1% DMSO for each representative quadrant (* p < 0.05; ** p < 0.01 and *** p < 0.001). Co-detection of MitoPotential dye and 7-AAD indicate intact mitochondria and cell death, respectively. The red dots indicate cell populations within a sample. Cells in the live quadrant (bottom left) are viable and have an intact mitochondrial membrane. These cells are negative for MitoPotential dye and 7-AAD. Cells in the depolarized/live quadrant (bottom left) are positive for MitoPotential dye only, indicating a depolarized mitochondrial membrane. The depolarized/dead quadrant (top left) represents cells that are positive for MitoPotential dye and 7-AAD, the structural integrity of these cells is damaged with a depolarized mitochondrial membrane. The dead quadrant (top right) represents dead cells with an intact mitochondrial membrane and are cells positive for 7-AAD only.