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. 2023 Oct 27;23:519. doi: 10.1186/s12870-023-04543-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Soybean triple mutation of EIL3, EIL4 and EIN2L generated by the CRISPR/Cas9 system in the T3 generation. A Sequences of WT and mutant plants at target sites EIL3-sg-T1, EIL4-sg-T2, and EIN2L-sg-T3. Dashes indicate deleted nucleotides. Proto-spacer adjacent motif (PAM) has been highlighted with red letters. B The sequence peaks of the wild-type (WT) and mutants at target sites EIL3-sg-T1, EIL4-sg-T2, and EIN2L-sg-T3. Red arrows indicate mutation locations. C Amino acid sequences of the triple mutation of EIL3, EIL4 and EIN2L and WT