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CTP-DB and -BL cells display a stemness phenotype. (A) Spontaneous spheroids were detected under an inverted light microscope in CTP-DB and -BL cultures. Magnification ×200, scale bar 50 μm. (B) Spheroids were observed in the chronically infected CTP-DB and -BL cell cultures in methylcellulose, scale bar 25 μm. (C) Confocal microscopic images of Nestin, Nanog, SOX2, and DAPI staining in CTP-DB and -BL cells. Uninfected PECs were used as controls; magnification ×63, scale bar 10 μm.
