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Effect of extracellular acidosis on PD-L1 expression in 4T1-Luc cells. The pH of the cell culture medium was adjusted by HCl or NaOH, and 4T1-Luc cells were incubated for 24 h and analyzed by flow cytometry. Flow cytometry dot plots (A) and mean fluorescence (B) of cell surface PD-L1 expression (* p < 0.05 for pH 6.5 vs. pH 7.4) and (C) PD-L1 mRNA levels analyzed by qPCR (* p < 0.05 for pH 6.5 vs. pH 7.4).
