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Bovine nucleus pulposus cultures remain stable after passaging. Primary cultures of bovine nucleus pulposus cells are shown, after harvest from caudal discs as described in Materials and Methods. High-power inverted microscope images are shown, panel (A) are cells at 60–70% confluence being grown up immediately after harvest (passage 0), and panel (B) are passage 10 cells at 80–90% confluence. Both panels show cells being cultured in normoxic conditions.
