Table A2.
Comparison of Current BMP-treatment Outcomes at 12 Weeks to Prior Experiments.
| Modality | p-Values for Comparisons | |||||||
|---|---|---|---|---|---|---|---|---|
| L4/5 Segment | L5/6 Segment | L4–6 Combined | ||||||
| Control | BMP | Control | BMP | Control | BMP | |||
| NIAD | 0.95 | 0.58 | 0.885 | 0.722 | 0.724 | 0.58 | ||
| Fusion Assessments | ||||||||
| Palpation | 1 | 0.285 | 1 | 0.458 | # | # | ||
| XR-Graded | 1 | 0.473 | 1 | 0.433 | # | # | ||
| XR-Categorical | 1 | 0.066 | 1 | 1 | # | # | ||
| Osteogenesis Quantifications | ||||||||
| XR-axitron | 1 | 0.019 * | 0.317 | 0.567 | 0.317 | 0.058 | ||
| Micro-CT | # | # | # | # | 0.806 | 0.327 | ||
| Stiffness Measurements were for the L4–6 Combined Segment | ||||||||
| Stiffness Assessments | IVAD | Four-Point Bending | ||||||
| Direction | Control | BMP | Control | BMP | ||||
| Extension | 0.001 * | 0.622 | 0.88 | 0.291 | ||||
| Flexion | 0.023 * | 0.622 | 0.174 | 0.159 | ||||
| Left | 0.001 * | 0.364 | 0.131 | 0.65 | ||||
| Right | 0.131 | 0.291 | 0.112 | 0.481 | ||||
| Coronal | 0.002 * | 0.496 | # | # | ||||
| Sagittal | 0.001 * | 0.573 | # | # | ||||
A comparison was made between multimodally assessed outcomes at lumbar level 4/5 (L4/5), lumbar 5/6 (L5/6) and these levels combined (L4–6) at 12 weeks following intradiscal injection of bone marrow stromal cells that were either negative control cells, or gene-programmed to express bone morphogenetic protein (BMP) BMP2/BMP7 heterodimers. Assessments were performed as described in Materials and Methods, prior data was from Cunningham et al (2022). Asterisk (*) denotes significant differences p ≤ 0.05 were observed in the comparisons, hashtag (#) indicates inability to make the comparison. The systematic differences observed for the IVAD negative control comparisons were thought due to differences in data dispersion, and the XR osteogenesis difference at L4/5 was lost when the data was not stratified by level.