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. 2023 Oct 19;13(10):939. doi: 10.3390/bios13100939

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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The promising future of go-FLIM development. (A) A schematic representation illustrating the proposed sensing mechanism of single-FP-based FLIM biosensors. A red circle indicates the rotation of chromophore. (B) Conceptual design of a single chemogenetic protein-based FLIM biosensor and its FLIM response. (C) Representation of multiplex imaging employing go-FLIMs that target various analytes across different organelles such as the mitochondria (mito), cytoplasm (cyto), and endoplasmic reticulum (ER). (D) Introduction of methodologies for the screening of go-FLIM. Illustrations were created with BioRender.com.