Table 2.

Reported single-FP-based FLIM biosensors for the detection of various targets.

Targets	Names	FP Reporters	2p-FLIM	𝜏free (ns)	𝜏bind (ns)	Δ𝜏(bind-free) (ns)	Ref.
NAD+/NADH	Peredox	cpT–Sapphire	Yes	2.63	1.87	−0.76	[42]
Glucose	iGlucoSnFR–TS	cpT–Sapphire	Yes	1.40	1.78	0.38	[44]
Lactate	LiLac	cp–mTQ2	Yes	3.00	1.80	−1.20	[46]
ATP	qMaLioffG	Citrine		2.57	1.49	−1.08	[40]
Acetylcholine	GRABACh3.0	cpGFP	Yes	3.34	3.51	0.17	[45]
Histidine	FHisJ	cpYFP		2.80	1.60	−1.20	[47]
Ca2+	Tq–Ca–FLITS	cp–mTQ2		1.40	2.78	1.38	[27]
	CatchER	EGFP		2.18	2.61	0.43	[48]
	RCaMP1h	cp–mRuby	Yes	-	-	1.10	[43]
pH	pHRed	mKeima–A213S	Yes	1.72 (pH 5)	2.12 (pH 8)	0.40	[13]
	SypHerRed	cp–mApple	Yes	0.72 (pH 6.9)	1.05 (pH 7.7)	0.33	[41]
	SypHer3s	cpYFP	Yes	1.20 (pH 6.5)	2.30 (pH 9.5)	1.10	[49]
H2O2	Hyper3	cpGFP		1.29	0.92	−0.37	[50]