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. 2023 Oct 11;24(20):15093. doi: 10.3390/ijms242015093

Table 2.

Overview of the indirect plasma treatment results in recent years.

Plasma Source Liquid PTS Characteristics Object Key Results References
DBD Distilled water pH: 3.42 ± 0.19 and 2.64 ± 0.05;
ORV: 461.67 ± 18.18 and 547.33 ± 9.02 mV;
Ec: 182.67 ± 16.8 and 883.33 ± 37.21 µs/cm;
[O3]: 1.21 ± 0.24 and 6.05 ± 0.73 mg/L;
[H2O2]: 181.67 ± 40.41 and 658.33 ± 28.87µM;
[NO3]: 4.03 ± 0.19 and 32.45 ± 5.43 mg/L;
[NO2]: 0.31 ± 0.04 and 0.46 ± 0.08 mg/L,
When exposed to 10 kHz and 200 Hz, respectively
Fresh-cut potato Disinfection.
Antioxidant properties.
Enzyme Inactivation.
[115]
DBD Deionized water pH: 7.17 ± 0.34, 8.04 ± 0.51, 3.97 ± 0.065, 6.807 ± 0.210;
ORP: 176.5 ± 3.53, 194 ± 5.65, 301 ± 1.41, 241.5 ± 0.70 mV;
TDS: 16.9 ± 1.27, 26.5 ± 1.31, 51.6 ± 1.44, 30.2 ± 1.74 ppm;
Ec: 21 ± 2.83, 33.7 ± 1.62, 74 ± 2.12, 43.3 ± 2.99 µS/cm
[NOx]: 0.52 ± 0.04, 0.452 ± 0.2, 130.61 ± 1.39, 144.37 ± 1.48 µM;
[H2O2]: 0.417 ± 0.01, 0.447 ± 0.03, 4.5 ± 0.26, 3.875 ± 0.441 µM for deionized water with Met+, deionized water with Met+, PAW without Met+, PAW with Met+, respectively
Pak Choi seeds (Brassica campestris L.) Faster germination.
Increased seedling length.
Higher chlorophyll and protein content.
Positive gene expression.
[116]
DBD Deionized water [NO3]: ~25, 35, 60, 90, 110 mg/L
[NO2]: ~0.15, ~0.4, 0.5, 0.82, 0.9 mg/L
[H2O2]: <1, ~1.5, 3.6, 4.0, 5.5 mM
[O2]: ~26, 34, 22, 7, 5 mM for 1, 2, 3, 4, 5 min. DBD treatment, respectively
Strawberry Longer shelf life, reduced spoilage.
No taste/texture change.
Best quality after 4 days of PAW treatment.
[117]
DBD Pure water pH: 5.16 ± 0.03 Button mushrooms (Agaricus bisporus) Decreased champignon browning, inhibited enzymes, and maintained organoleptic quality for preservation. [118]
DBD Deionized water [NO3]: ~0.1, 0.2, 0.4, 0.7, 1.9 mM
for 3, 5, 10, 15, 30 min. 12 kHz plasma treatment, respectively
[NO3]: ~0.25, 0.25, 1.2, 1.3, 3.3 mM For 3, 5, 10, 15, 30 min. 20 kHz plasma treatment, respectively
Arabidopsis thaliana seedlings Low DBD-PAW doses influenced intracellular Ca2+ signals. [119]
DBD Distilled water [H2O2]: 0, 0, 180.4 ± 7.2, 294.9 ± 18.4, 387.7 ± 24.5 μM
[·OH]: 16.7 ± 3.3, 25.5 ± 2.9, 55 ± 4.0, 80.0 ± 2.9, 0 μM
[NO2]: 0, 0, 0, 0, 0 μM
[NO3]: 0, 0, 22.7 ± 3.6, 18.1 ± 3.8, 15.7 ± 1.9 μM
With He/O2 plasma activation times of 10, 15, 30, 45, and 60 min, respectively
[H2O2]: 0, 0, 47.8 ± 3.3, 33.3 ± 2.0, 30.2 ± 0.2 μM
[·OH]: 14.5 ± 3.3, 23.1 ± 2.3, 42.7 ± 5.2, 54.3 ± 4.7, 0 μM
[NO2]: 0, 0, 56.5 ± 2.5, 47.3 ± 1.7, 35.4 ± 4.0 μM
[NO3]: 0, 0, 3420.7 ± 103.5, 4948.6 ± 74.5, 6191.1 ± 101.2 μM
With air plasma activation times of 10, 15, 30, 45, and 60 min, respectively
Nicotiana tabacum ‘Havana 425’ seeds Air and He/O2 PAW enhanced testa and endosperm rupture in low fluence conditions.
Increased GA3ox2 and EXPA4 activity.
[120]
DBD Distilled water pH: ~5.8, 5.45, 4.8, 4.85, 4.8, 4.8
Ec: ~10, 13, 14, 15, 18, 17 μS/cm
[H2O2]: ~15, 19, 33, 40, 52, 69 mg/L
[NO2]: <5 mg/L
[NO3]: ~20, 30, 40, 38, 50, 58 mg/L
For 5, 10, 15, 20, 25, and 30 min plasma treatment, respectively
Lettuce (Lactuca sativa L.) seeds Boosted lettuce seed germination with positive effects on seedling growth and chlorophyll content. [121]
DBD Deionized water pH: 3.62 ± 0.02, 3.34 ± 0.03, 2.94 ± 0.08, 2.62 ± 0.07, 2.37 ± 0.04
Ec: 118.10 ± 2.26, 218.50 ± 9.64, 460.33 ± 15.25, 972.93 ± 32.41, 1847.00 ± 70.19 µS/cm
[H2O2]: 0.09 ± 0.01, 0.14 ± 0.01, 0.27 ± 0.02, 0.88 ± 0.04, 1.31 ± 0.04 mg/L
[NO2]: 1.09 ± 0.11, 1.24 ± 0.12, 1.85 ± 0.07, 3.68 ± 0.12, 5.17 ± 0.16 mg/L
[NO3]: 25.29 ± 2.88, 49.05 ± 2.61, 102.67 ± 6.30, 204.87 ± 8.74, 389.08 ± 12.24 mg/L
For 5, 7, 12, 19, and 40 min plasma treatment, respectively
Arabidopsis thaliana L. Affected root hair density via gene regulation (COBL9, XTH9, XTH17). [122]
DBD Distilled water [H2O2]: 0.7 ± 0.2 mg/L
[NO2]: 1.071 ± 0.005 mg/L
[NO3]: 24.7 ± 2.3 mg/L
Maize (Zea mays L. ‘SY ORPHEUS’) Reduced leaf chlorophyll, changed fluorescence parameters, and increased nitrogen content. [123]
DBD Distilled water pH: 6.7
Ec: 34 µS
[H2O2]: 1.4 ± 0.4 mg/L
[NO2]: 0.753 ± 0.009 mg/L
[NO3]: 20.4 ± 1.8 mg/L
Soil Minimal effects on soil, with slight changes in evaporation, pH, and water absorption.
Higher PAW doses slowed tap water absorption but increased water retention.
[124]
DBD Deionized water pH: 4.3
[NO3]: 25.7 mg/L
[NO2]: 16.4 mg/L
[H2O2]: 2–5 mg/L
Xylella fastidiosa Deactivation of Xylella fastidiosa cells. [77]
DBD Water [H2O2]: 1,1,2,2,10,100, 100, >100, >100 ppm for 30 kV, 3 min., 30 kV, 5 min., 30 kV, 7 min., 50 kV, 3 min., 50 kV, 5 min., 50 kV, 7 min., 70 kV, 3 min., 70 kV, 5 min., 70 kV, 7 min. plasma treatment, respectively. Soybeans (Glycine max) Faster germination and growth. ZnO nanoparticles reduced heavy metal uptake in plants. [125]
APPJ Deionized water pH: ~4.5, 3.5, 3
Ec: ~50, 270, 590 µS/cm
For 10, 15, and 20 kV treatment, respectively
Cuimi kumquat Reduced pesticide residues, preserved soluble solids, increased acidity, and maintained fruit color. [126]
Microwave-driven plasma torch Deionized water [NO3]: 72.3 mg/L
[NO2]: 1600.7 mg/L
[H2O2]: 717.3 mg/L
Pseudomonas fluorescence suspended cells and P. fluorescence biofilms Strong antibacterial effects. Effective against suspension cells and P. fluorescence biofilms. [127]
APPJ Reverse osmosis water [NO3]: 42.7 ± 0.70 mg/L
[NO2]: 14.7 ± 0.58 mg/L
pH: 3.17 ± 0.06
Ec: 311.7 ± 12.01 µS/cm
ORP: 554 ± 2.65 mV
Water spinach (Ipomoea aquatica) seeds Phenols increase with PAW, more with Cd, and less with Pb.
Flavonoids rise with PAW and Cd, with no change with Pb. Heavy metals impact PAW effects.
[128]
Pinhole plasma jet Tap water pH: 5.5–6
Ec: 1.5 µS/cm
[NO3]: 883.59 mg/L
[NO2]: 31.56 mg/L
[H2O2]: 102.99 mg/L
Green oak lettuce (Lactuca sativa L.) Growth parameters are mostly unaffected.
Leaf area and greenness differed.
Yields similar to commercial nitrate.
Lower nitrate residues with PAW treatment.
Plasma nitrate is converted to amino acids at higher concentrations than normal nitrate.
[129]
Microwave-driven plasma torch. Deionized water pH: ~4
ORP: ~502 mV
Ec: ~1367 µS/cm
[NOx]: 4000 µM
Escherichia coli K-12 (KCTC 1116), Pseudomonas aeruginosa (KCTC 1636), Staphylococcus aureus (ATCC 12600) Reduced viable cells, shifted gene expression (soxRS up, oxyR down), leads to nitric oxide accumulation, and alters bacterial cell morphology. [130]
APPJ Commercially purified water of pharmaceutical degree (Pharmacopoeia Europea, Ph. Eur. 9) pH: 6.1, 6.1, 5.8
Ec: ~27 µS/cm
[H2O2]: 4.1, 3.1, 0 mg/L
[NO2]: 3.3, 2.8, 1.4 mg/L
[NO3]: 11.2, 5.5, 5.4 mg/L
For 10, 20, 30 min after plasma treatment, respectively
Lettuce (Lactuca sativa L.) Better growth on day 7, but advantages faded by days 14, 21, and 28.
No significant root system impact. Increased dry matter content.
[131]
APPJ Commercially purified water of pharmaceutical degree (Pharmacopoeia Europea, Ph. Eur. 9) pH: 6.1, 6.1, 5.8
[H2O2]: 4.1, 3.1, 0 mg/L
[NO2]: 3.3, 2.8, 1.4 mg/L
[NO3]: 11.2, 5.5, 5.4 mg/L
For 10, 20, and 30 min after plasma treatment, respectively
Sweet pepper seeds (Capsicum annuum): ‘Bibic’ and ‘Bernita’ Height, weight, leaf count, interleaf nodes, and buds increased.
Dry matter content rose.
Yield increased.
[132]
Gliding arc plasma jet Tap water, deionized water, distilled water, filtered water, and 0.9% saline Plasma-treated tap water:
pH: 5.56, 3.55, 3.06, 2.57 ± 0.09
ORP: 83, 193, 221, 250 ± 5.0 mV
TDS: 50, 160, 330, 720 ± 5.0 ppm
Ec: 70, 220, 470, 720 ± 5 μS/cm
[H2O2]: 15.0, 80.3, 127.3, 111.7 mg/L
[NO2]: 25.0, 47.6, 48.6, 55.6 mg/L
[NO3]: 24.0, 53.3, 120.4, 69.3 mg/L
Plasma-treated deionized water:
pH: 4.00, 3.30, 2.95, 2.47 ± 0.09
ORP: 169, 208, 228, 239 ± 5.0 mV
TDS: 40, 170, 450, 560 ± 5.0 ppm
Ec: 50, 240, 310, 800 ± 5 μS/cm
[H2O2]: 9.3, 76.5, 82.0, 160.3 mg/L
[NO2]: 9.2, 46.2, 45.8, 62.0 mg/L
[NO3]: 7.3, 47.4, 106.3, 69.3 mg/L
After 1.0, 5.0, 30.0, and 60.0 min., respectively
Escherichia coli
Oral keratinocyte cell cultures
Strong antimicrobial efficacy.
Low cytotoxicity on oral keratinocytes.
[133]
APPJ Acidic electrolyzed water pH: ~2.3, 2.5, 1.95, 1.91
ORP: ~605, 608, 610, 855 mV
Ec: ~1600, 2470, 2750, 3200 μS/cm
[H2O2]: 75, 82, 75, 72 mg/L
[NO2]: ~85, 90, 90, 90 mg/L
[NO3]: 2.02, 75.28, 138.7 and 219.6 mg/L
With plasma activation times of 3, 6, 10, and 15 min, respectively
B. subtilis (ATCC6633) and E. coli (ATCC8739) Plasma-activated acidic electrolyzed water (PA-AEW) effectively kills B. subtilis, surpassing PAW and AEW. [134]
Pinhole plasma jet Deionized water [H2O2]: 369.12 mg/L Escherichia coli and Colletotrichum gloeosporioides in chili (Capsicum annuum L.) Carbendazim and chlorpyrifos reduction. 100% fungal spore germination inhibition. [135]
APPJ Ultrapure milli-Q pH: 6.5, 6.0
Ec: 99.0 ± 13.5, 177.3 ± 11.0 µS/cm
ORP: 423.3 ± 11.7, 483.3 ± 12.6 mV
TDS: 36.3 ± 2.1, 68.3 ± 5.5 ppm
[O3]: 2.8 ± 0.1, 2.1 ± 0.3 mg/L
[H2O2]: 0.5 ± 0.1, 1.5 ± 0.2 mg/L
[NO2]: 5.1 ± 0.4, 10.5 ± 1.1 mg/L
[NO3]: 34 ± 2.6, 53.9 ± 3.6 mg/L
For 5- and 10-min plasma treatment, respectively
Pea (Pisum sativum L.) seeds PAW-treated pea seeds had improved germination, growth, and biochemical traits.
Wax removal, increased hydrophilicity, and enhanced antioxidant enzyme activity.
[136]
APPJ Deionized water
and deionized water
+Mg2+
For PAW-Mg2+
pH: ~4.8, 5.2, 5.8, 6.0, 6.6
[NO3]: ~25, 40, 40, 50, 60 mM
For PAW
pH: ~3.6, 3.2, 2.6, 2.2, 2.3
[NO3]: ~20, 40, 80, 120, 120 mM
For 3, 6, 10, 15, and 20 min plasma treatment, respectively
Cryptococcus pseudolongus Magnesium-enriched PAW controls nitrate and exhibit antifungal activity against C. pseudolongus, with nitrate capture influencing its effectiveness. [137]
APPJ Ultrapure milli-Q pH: ~2.5, 2.2, 2.0, 1.5, 1.5, 1.0
Ec: ~1, 3, 9, 22, 32, 39 mS/cm
[NO3]: ~200, 300, 500, 800, 900, 1200 mg/L
[NO2]: ~1500, 3000, 10,000, 14,000, 16,000, 16,000 µg/L
[O3]: ~26, 24, 24, 17, 15, 14 mg/L
[H2O2]: ~6, 4.5, 4.5, 3, 2.5, 2 mg/L
For ORP 590, 630, 640, 700, 760, and 795 mV, respectively
C. albicans,
Citrus limon
Reduced C. albicans by damaging cell membranes. Long-term fungicidal effects on C. albicans and Citrus limon spoilage fungi. [138]
APPJ Distilled water pH: 6.50 ± 0.07
[O3]: 0.45 ± 0.01 mg/L
[H2O2]: 8.75 ± 0.09 mg/L
[NO2]: 6.00 ± 0.06 mg/L
[NO3]: 46.00 ± 0.47 mg/L
Paddy seeds (Oryza sativa L.‘BRRIdhan 28’) Enhanced rice seed germination, improved plant growth, defense mechanisms, enzymatic activity, protein, sugar content, and yielding. [139]
APPJ Reverse osmotic water pH: 6.79 ± 0.18, 4.22 ± 0.07, 3.37 ± 0.06, 3.17 ± 0.06
Ec: 104.9 ± 22.55, 117.9 ± 2.80, 208.3 ± 6.51, 311.7 ± 12.01 µS/cm
ORP: 370 ± 7.51, 465 ± 9.29, 534 ± 13.0, 554 ± 2.65 mV
[H2O2]: ~ 78, 142, 115, 95 mg/L
[NO2]: 10.3 ± 0.58, 17.3 ± 1.15, 13.0 ± 1.00, 14.7 ± 0.58 mg/L
[NO3]: 13.8 ± 0.15, 29.8 ± 1.67, 33.3 ± 0.87, 42.7 ± 0.70 mg/L
For 5, 10, 15, and 20 min plasma treatment, respectively
Water spinach (Ipomoea aquatica) seeds Reduced Cd uptake in spinach but not Pb. [140]
APPJ Sterile deionized water pH: 3.53, 3.24, 3.10, 3.01
[H2O2]: ~25, 50, 60, 55 µM
[NOx]: ~0.9, 1.7, 2.5, 2.7 mM
For 1, 3, 6, and 10 min by the soft plasma jet treatment, respectively.
Aspergillus brasiliensis Damaged cell walls and reduced spore viability [141]
APPJ Deionized water pH: 4.3 ± 0.3, 4.7 ± 0.2, 5.1 ± 0.3, 6.2 ± 0.4
[NOx]: 490.0 ± 53.7, 520.7 ± 71.6, 450.1 ± 69.5, 597.5 ± 53.4 M
[NH3]: 2.1 ± 0.1, 2.6 ± 0.3, 2.9 ± 0.2, 4.9 ± 0.3 mg/L
[H2O2]: 38.2 ± 5.0, 35.3 ± 3.1, 32.6 ± 6.5, 28.7 ± 4.2 M
For PAW, Zn- PAW, Al- PAW, and Mg- PAW, respectively.
Maize plants (Zea mays L.) PAW with added Mg, Al, or Zn ions accelerated germination and stem growth.
Metal ions enhanced nitrogen reduction, leading to increased chlorophyll and protein content in plants.
[142]
Plasma torch Deionized water pH: ~3
[H2O2]: ~0.5 mg/L
[NO2]: ~47 mg/L
[NO3]: ~33 mg/L
Arabidopsis thaliana PAW exposure rapidly increased Ca2+ in cells.
Nitrates, nitrites, and hydrogen peroxide at PAW concentrations did not affect Ca2+ dynamics.
[143]
Plasma torch Distilled water pH: ~3
[H2O2]: 70 µM
[NOx]: 15 mM
Strawberry seeds Improved germination [144]
Plasma torch Distilled water pH: ~3
[H2O2]: 22.8 µM
[NOx]: 5.7 mM
Cotton, wheat, and strawberry seeds Improved germination.
Protection against fusarium and hyperthermia.
PAW is better than commercial seed germination stimulators.
[145]
Corona discharge Distilled water pH: 3.3.
[H2O2]: 4.5 ± 0.1
[NO2]: 30.4 ± 0.9
[O3]: 0.3 ± 0.1 mg/L
Fresh rocket (Eruca sativa) leaves Preserved nutrients, increased some vitamins and polyphenols.
Decreased antioxidant and catalase activity, aiding storage quality.
[146]
Corona discharge Distilled water [H2O2]: 97.4 ± 3.2 mg/L
[NO3]: 391.1 ± 9.3 mg/L
[O3]: <3.8 mg/L,
[NO2]: <10−3 mg/L of NO2 mg/L
Wheat grains (Triticum aestivum L.),
Escherichia coli,
Saccharomyces cerevisiae
Improved germination, shoot growth, and decontamination of E. coli and S. cerevisiae. [147]
Corona discharge Distilled water pH: 3.3
[H2O2]: 4.5 ± 0.1 mg/L
[NO2]: 30.4 ± 0.9 mg/L
[O3]: 0.3 ± 0.1 mg/L
Fresh-cut Eruca sativa Reduced radical scavenging activity over time but increased total phenols and glucosinolate percentage.
PAW polyphenol extract showed no cytotoxicity and improved redox balance.
[148]
Gliding arc plasma Tap water pH: 3.4, 3.3
TDS: 90, 110 mg/L
Ec: 190, 230 μS/cm
[H2O2]: 0.01, 0.028 mM/L
[NO3]: 0.156, 0.176 mM/L
For 5 and 15 min after plasma treatment, respectively
Maize (Zea mays L.) seeds 15 min treatment led to 100% seed germination and improved stem length, leaf width, collar diameter, chlorophyll content, and water uptake. [149]
Gliding arc plasma Distilled water pH: ~4.2 ± 0.2, 3.7 ± 0.1, 3.3 ± 0.3
[H2O2]: ~6 ± 1, 7 ± 3, 12 ± 5 µM
[NO2]: ~1.9 ± 0.4, 2.4 ± 0.3, 2.9 ± 0.6 mM
For 5, 10, and 20 min plasma treatment, respectively
Beetroot (Beta vulgaris ‘CYLINDRA’ ), carrot (Daucus carota ‘AFALON F1’) seeds Improved germination in Beta vulgaris and had mixed effects on Daucus carota seeds compared to NaOCl, also altering fungal species composition. [150]
Gliding arc plasma Nutrient solution [NO3]: 191.9 ± 3.1, 189.8 ± 1.7, 191.6 ± 1.6
[NO2]: 18.8 ± 1.9, 18.6 ± 0.9, 18.7 ± 1.1, For one-time DBD processing and multiple processing, respectively
Ocimum basilicum L. Boosted basil growth, enriched aroma compounds, and reduced algae. [151]
Gliding arc plasma Deionized water pH: 4.69 ± 0.08, 4.48 ± 0.05, 4.37 ± 0.07, 4.29 ± 0.06
T: 16.5 ± 0.04, 16.59 ± 0.06, 16.62 ± 0.03, 16.64 ± 0.04 °C
Ec: 50.2 ± 1.60; 60.2 ± 1.20, 70.6 ± 1.80, 76.8 ± 1.60 µS/cm
[NO2]: 0.49 ± 0.04, 0.60 ± 0.03, 0.80 ± 0.06, 0.86 ± 0.04 mg/L
[NO3]: 0.49 ± 0.14, 4.84 ± 0.12, 6.69 ± 0.16, 7.20 ± 0.12 mg/L
[NH3]: 1.11 ± 0.07, 2.68 ± 0.09, 3.01 ± 0.07, 3.40 ± 0.08 mg/L for 5, 10, 15, 20. GAD treatment, respectively
Phapar (Fagopyrum esculentum), barley (Hordeum vulgare), mustard (Brassica nigra), and rayo (Brassica juncea) Improved seed germination, uniformity, daily germination, increased water uptake, root/shoot length, and seed vigor. [152]
RF discharge Distilled water pH: 3.0
[H2O2]: 100 ppm
Flour from Noui Khuea brown rice (Oryza sativa L.) conjugated with three different phenolic compounds—gallic acid, sinapic acid, and crude Mon-pu (Glochidion wallichianum Muell Arg) extract Gallic acid boosts 1,1-diphenyl-2-picrylhydrazyl removal in starch, especially when PAW-synthesized, regardless of ultrasound.
Complexation index, resistant starch lower than crude Mon-pu extract complex.
[153]
Direct discharge Distilled water pH: 3.3
[O3]: 0.3 ± 0.1 mg/L
[H2O2]: 4.5 ± 0.1 mg/L
[NO2]: 30.4 ± 0.9 mg/L
Eruca sativa Mill β-sitosterol and campesterol decreased, while β-carotene, luteolin, and chlorophyll b increased after 2-min PAW exposure.
Chlorophyll content reduced at 20 min.
[154]
Direct discharge Distilled water pH: 3.3
[O3]: 0.3 ± 0.1 mg/L
[H2O2]: 4.5 ± 0.1 mg/L
[NO2]: 30.4 ± 0.9 mg/L
Eruca sativa Reduced bacteria by 1.7–3 Log CFU/g with minor quality changes, surpassing the antibacterial effect of hypochlorite. [155]
Transient spark discharge Tap water pH: 7.5
[H2O2]: 0.5 ± 0.1 mM
[NO2]: 0.6 ± 0.1 mM
[NO3]: 1.7 ± 0.3 mM
Corns of maize (Zea mays L.) (hybrid Bielik) Enhanced seedling growth. It affected chlorophyll, carotenoid levels, and leaf arsenic accumulation, not root accumulation. [156]
Glow discharge Tap water [H2O2]: ~0.33, 0.64, 1.00 mM
[NO2]: ~0.93, 0.59, 0.95 mM
[NO3]: ~2.46, 1.40, 2.34 mM
For transient spark, PAW of glow discharge at activation time 1 min and 2 min, respectively.
Dried barley (Hordeum vulgare L. ‘Kangoo’) grains and pea (Pisum sativum L. ‘Eso’) seeds Enhanced pea growth and amylase activity without harm, while barley suffered DNA damage, growth reduction, and oxidative stress. [157]
Glow discharge. Re-distilled water
and the inorganic salt concentration (ammonium nitrate, NH4NO3) in the solution turned out to be 0.50% (m/w)
Dickeya solani IFB0099, Pectobacterium atrosepticum IFB5103 strains Bactericidal effects on Dickeya solani and Pectobacterium atrosepticum within 24 h. [158]
Transient spark Tap water pH: ~ 7.5
[H2O2]: ~ 0.42 mM
[NO3]: ~ 0.85 mM
Lettuce (Lactuca sativa L. var. capitata ‘Král máje I’) PAW-irrigated lettuce had similar dry weight but higher pigment content, photosynthetic rate, and lower antioxidant enzyme activity compared to H2O2 + NO3 irrigation. [159]
Glow discharge Tap water Poa pratensis, Lolium perenne Decreased fungal diseases, improved turf density and overwintering.
Synergistic effect with bio-stimulant.
[160]
Spark discharge Deionized water pH: 6.7, 6.4, 6.0, 5.3, 3.7
[H2O2]: ~60, 75, 100, 150, 240 µM
[NO2]: 25, 30, 50, 75, 230 µM
For 3, 6, 9,12, and 15 min plasma treatment, respectively.
Black gram (Vigna mungo L.) Increased H2O2 and ROS levels in seeds, leaves, and roots.
Elevated catalase levels linked to VmCAT gene activation.
[161]
Glow discharge 80 mM L-phenylalanine [H2O2]: 282 µM
[NO2]: 4.4 µM
[NO3]: 520 µM after 4 min plasma treatment
Raphanus sp.,
E. coli O1:K1:H7
Seedlings stimulation and antibacterial effect. [162]
T-shaped reactor Distilled water pH: 4.6; 4.4; 5.1; 4.1; 5.1; 3.9; 5.5; 3.8
[NO3]: 30, 40, 14, 49, 15, 55, 8, 68 ± 10% mg/L
[H2O2]: 7, 8, 3, 13, 5, 14, 1, 22 ± 5% mg/L
During treatment in the modes:
Frequency: 150, 250, 60, 250, 60, 250, 250, 250 Hz;
Duration: 2, 2, 2, 2, 1.5, 1.5, 2, 2 ms;
Airflow: 1, 1, 1, 1, 1, 1, 2.5, 2.5 L/min;
Liquid flow: 10, 10, 20, 3.33, 25, 3.33; 85, 4 L/min, respectively
Wheat (Triticum aestivum) grains Reduced mitotic activity in wheat sprouts’ cells.
Genotoxicity tied to H2O2, NO3 levels. Positive effect on wheat germination
[163]
Underwater discharge Distilled water pH: ~3.91 ± 0.03,
[O3]: ~0.25 ± 0.01 mg/L
[H2O2]: ~7.50 ± 0.05 mg/L
[NO2]: ~4.59 ± 0.04 mg/L
[NO3]: ~26.58 ± 0.25 mg/L
Potato (Solanum tuberosum L.) PAW spraying on potato foliage enhanced growth, enzyme activity, protein and sugar content, and tuber yield. [164]
Underwater discharge Distilled water with KH2PO4 [H2O2]: 100 μM Spring spelled seeds,
spring rye seeds
Improved germination,
improved root development.
[165]
(1) Underwater discharge.
(2) Plasma torch
(1) Distilled water + KCl
(2) Distilled water
(1) pH: 8.3
[H2O2]: 7.12 mM
[NOx]: 22.05 mM
(2) pH: 4.5
[H2O2]: 0.11 mM
[NOx]: 87 mM
Apple tree Increased primary nutrient content in fruits and leaves.
Ca in the fruits increased significantly.
[166]
Underwater discharge Distilled water + KCl pH: 8.3
[H2O2]: 7.12 mM
[NOx]: 22.05 mM
Sorghum and barley seeds, strawberry crops Improved germination and crop development.
Increased drought resistance.
[167]