Table 1.

Statistical data of back-calculated concentrations of PACAP, from 10 independent runs using six calibration points.

Expected Concentration (pmol/L)	Observed Concentration (pmol/L)			Accuracy (Observed/ Expected) × 100 (%)
	Mean	SD	%CV
400	404	4.3	1.1	101
200	195	4.0	2.0	97.6
80	85.2	4.0	4.7	107
32	32.2	2.6	8.0	101
12.8	13.5	1.1	8.2	105
5.12	3.84	0.6	15.7	75.1

CV: Coefficient of variation; SD: standard deviation, n = 10 plates.