Skip to main content
. 2023 Sep 27;14(10):1880. doi: 10.3390/genes14101880

Figure 1.

Figure 1

GSEA of 161 pediatric cases of ALL identified Cancer Hallmarks associated with higher circulating WBCs. (a) List of Hallmarks enriched in ALL blasts at diagnosis in samples with increased circulating WBCs (and thus associated with a worse prognosis) following MTX therapy. Highlighted is the topmost enriched pathway, TNF-α signaling via NF-κB, whose enrichment plot is shown in (b). (c,d) Expression levels of NFKB1 and REL in the top (n = 40) and bottom (n = 40) quartiles with the poorest (poor) and best (good) clinical responses to MTX. p-values for the unpaired t-test. Analysis conducted on data from Sorich et al. [25] Abbreviations: ALL—acute lymphoblastic leukemia; ES—enrichment score; FDR q-val—false discovery rate q-value; FWER p-val—familywise error-rate p-value; GSEA—gene set enrichment analysis; MTX—methotrexate; NES—normalized enrichment score; Size—the number of genes that comprise the Hallmark; NOM p-val—nominal p-value; WBCs—white blood cells.