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. 2023 Oct 13;24(20):15140. doi: 10.3390/ijms242015140

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Empirical confirmation demonstrates that the selected IRGs (LRP10, PGK1, and RPLP0) were suitable to be used in expression analyses in human PPAT obtained under different experimental conditions. Expression levels of (a) LEP, (b) ANGPT1, (c) MMP2, and (d) MMP9 before normalization (raw data) and after normalization with selected IRGs (LRP10, PGK1, and RPLP0) are shown. Normalization of genes was performed using a normalization factor obtained with the GeNorm software (v3.5) based on the expression levels of LRP10, PGK1, and RPLP0. Asterisks (* p < 0.05; ** p < 0.01, and *** p < 0.001) indicate statistically significant differences between groups.