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. 2023 Oct 13;24(20):15136. doi: 10.3390/ijms242015136

Figure 3.

Figure 3

Depletion of TKS4 and/or CD2AP expression in HCT116 colon cancer cells. (A) Wound healing assay in CD2AP-, TKS4-, and CD2AP/TKS4-silenced HCT116 cells, and the “% of gap area” was calculated 24 h after scratching. Dots indicate the percent of gap area of three replicates from each group. Between-group comparisons were performed using one-way ANOVA and the Benjamini–Hochberg FDR (false discovery rate) procedure and statistical significance was set at * p < 0.05. (n = 3); scale bar = 100 µm; gap area (µm). (B) EMT marker proteins (E-cadherin and vimentin) level as measured via WB (n = 3), validation of efficient CD2AP and TKS4 silencing and densitometry analysis of the marker proteins relative to GAPDH level. Dots indicate the values from three independent experiments. Between-group comparisons were performed using one-way ANOVA and the Benjamini-Hochberg FDR procedure, and statistical significance was set at * p < 0.05. (n = 3). (C) qPCR analyses of EMT markers in siTKS4-, siCD2AP- and siCD2AP/TKS4-double silenced HCT116 cells. Results are shown as fold change relative to the control. Dots indicate the values from three biological replicates. Between-group comparisons were performed using one-way ANOVA and Benjamini–Hochberg FDR procedure, and statistical significance was set at * p < 0.05, (n = 3).