Figure 1.

Schematics of the hybrid hydrogel generation. ECM hydrogel was made from omental tissue, which can be easily extracted from patients. Cells and ECM were then separated. Pluripotent stem cells were induced, while the ECM was processed into a thermoresponsive hydrogel. Using carbodiimide activation, YIGSR peptides were conjugated to alginate, which was integrated with the ECM hydrogel to create an ECM-based hydrogel with enhanced mechanical properties and endothelial cell-specific adhesion motifs.