Figure 2.

Etridiazole exposure-induced cardiac toxicity in zebrafish embryos/larvae. (A) Representative images showing pericardial edema (red dotted lines) and hyperemia (red arrow) in etridiazole-treated zebrafish embryos/larvae. Graphs showing the percentage of (B) edema symptoms, (C) pericardial edema, (D) embryos that showed reduced blood flow, and (E) hyperemia observed at designated time points. (F) Graph showing average heartbeats per minute scored at 48, 72, and 96 hpf in untreated and etridiazole-treated zebrafish embryos/larvae. The results are presented as mean ± SD. Unpaired Student’s t-test was applied for statistical significance evaluation. * Statistically significant (p < 0.05). UTZF, untreated zebrafish; ETZF, etridiazole-treated zebrafish. Scale = 0.5 mm.