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Purification of LipP from E. coli C600 harboring pPL2-1
| Purification method | Total protein (mg) | Total activity (Ua) | Sp act (U/mg) | Purifi- cation (fold) | Yield (%) |
| Cell extract | 180 | 5,200 | 29 | 1 | 100 |
| DEAE-Cellulofine | 5.3 | 1,300 | 250 | 8.6 | 25 |
| Gigapite | 0.8 | 860 | 1,100 | 38 | 17 |
Activity was measured by determination of 2,3-dimercaptopropan-1-ol formed from 2,3-dimercaptopropan-1-ol tributyl ester with 5,5′-dithio-bis(2-nitrobenzoic acid). The definition of unit is given in the text.
