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. 2023 Oct 16;24(20):15236. doi: 10.3390/ijms242015236

Figure 2.

Figure 2

The apoptotic effect of gallic acid in PANC-1 and MIA PaCa-2. (A) The occurrence of cell death in PANC-1 and MIA PaCa-2 cell lines was verified by the utilization of a confocal microscope. The nucleus exhibits blue fluorescence at a wavelength of 465 nm, while fluorescent green images are observed in dead cells at a wavelength of 520 nm. (B,C) The graph depicts the relationship between the total number of cells and the number of cells that exhibit positive staining for the TUNEL assay. Scale bar: 50 μm. (D,E) The effect of gallic acid (75 μM and 150 μM) on the distribution of the cell cycle in PANC-1 and MIA PaCa-2 cells was assessed using fluorescence-activated cell sorting (FACS). (F,G) The graph represents the ratio between the duration of M1 (sub G1) phase and M4 (G2) phase in the cell cycle. The aforementioned figures represent the mean values derived from three distinct experimental trials. Data represented means ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001 versus untreated group.