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The schematic of the transcription elements on the plasmid to optimize expression of recombinant proteins in E. coli and B. subtilis. These elements include: (1) origin of replication (ori); (2) translation initiation sequence: a ribosomal-binding site (RBS) and start codon; (3) promoter; (4) affinity tag; (5) multiple cloning site (MCS); (6) transcription termination sequence; (7) selectable marker.
