Figure 1.

GnRH-II induces decidual stromal cell motility. (A) Human decidual endometrial stromal cells were seeded by applying the Transwell migration assay. The GnRH-II agonist promoted decidual stromal cell migration through the uncoated porous filter in a dose-dependent pattern at 1 nM to 1 μM concentrations, with the most significant effect at 1 μM. (B) Decidual endometrial stromal cells were seeded onto a Matrigel-precoated filter in the Transwell chambers with or without the GnRH-II agonist (1 nM to 1 μM concentrations, as indicated). After culturing for 24 h (migration) and 48 h (invasion), we removed cells in the upper side of the filter and fixed, stained, and counted the migrated or invaded cells. Low, representative pictures. Columns, the mean number of migrated or invaded cells of five field triplicate wells from three independent experiments; bars, SE; * p < 0.05, versus control. Scale bars represent 50 μm.