Skip to main content
. 2023 Oct 17;24(20):15271. doi: 10.3390/ijms242015271

Figure 2.

Figure 2

The GnRH-II-stimulated migration and invasion of decidual endometrial stromal cells (ESC) through GnRH-I receptors in humans. (A) Immunoblot assay was applied to identify GnRH-I receptor levels in human decidual endometrial stromal cells. Human decidual endometrial stromal cells were transfected by human si-GnRH-IR or scrambled siRNA (si-Ctrl) with Lipofectamine RNAiMAX for one day. Negative control was compared by HeLa cells. (B) The impacts of si-GnRH-IR transfection on GnRH-II-stimulated cell migration. GnRH-II (1 μM) was used to treat the pre-transfected cells with si-GnRH-IR for 24 h. A migration assay was applied to evaluate cell motility. The results are shown as the mean ± SEM from three individual experiments (* p < 0.05, versus control; # p < 0.05, versus GnRH- II). (C) The impacts of si-GnRH-IR transfection on GnRH-II-induced cell invasion. GnRH-II (1 μM) was used to treat the pre-transfected cells with si-GnRH-IR for 48 h. The invasion assay was utilized to assess cell motility. The results are shown as the mean ± SEM from three individual experiments (* p < 0.05, versus control; # p < 0.05, versus GnRH-II).