Skip to main content
. 2023 Oct 17;24(20):15271. doi: 10.3390/ijms242015271

Figure 4.

Figure 4

GnRH-II and EGF cotreatment to enhance the cell motility of decidual stromal cells. (A) Human decidual endometrial stromal cells were individually treated with 100 ng/mL of EGF, 100 nM of GnRH-II, and in combination for 24 h and then seeded by applying the Transwell migration assay. (B) Human decidual endometrial stromal cells were seeded onto a Matrigel-precoated filter in the Transwell chambers with 100 ng/mL of EGF, 100 nM of GnRH-II, and in combination for 48 h. We removed harvested cells in the upper side of the filter after culturing and then fixed, stained, and calculated the migrated or invaded cells. Low, representative pictures. Columns, the mean number of migrated or invaded cells of five field triplicate wells from three independent experiments; bars, SE; * p < 0.05, versus control. Scale bars represent 50 μm.