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. 2023 Sep 30;14(10):797. doi: 10.3390/insects14100797

Figure 6.

Figure 6

PCR-generated sequences from intron 7 of cSHMT aligned with sequences from GenBank. The sequences 7–12 were generated as part of this study except 11 which is from Helicoverpa zea NFMG01027888.1. The sequences are as follows: (1) Anopheles gambiae; (2) ORF Hz2V066; (3) Bombyx mori; (4) Trichoplusia ni; (5) Spodoptera litura; (6) H. armigera; (7) H. zea heterozygote light band; (8) H. zea × H. armigera lab reared light band; (9) H. zea heterozygote heavy band; (10) H. zea × H. armigera lab reared heavy band; (11) H. zea; and (12) H. zea field collected. Sequences from hybrids and heterozygotes were separated by gel electrophoresis, excised, and purified. Black boxes indicate TE flanking direct repeats and blue boxes indicate inverted repeats. H. zea sequence 11 was manually adjusted to align flanking direct repeats, the 5′ and 3′ ends of the alignment were trimmed to fit the figure.