Table 1.
Primers used in this study.
| Name | Description | Sequence | Tm (°C) | Source |
|---|---|---|---|---|
| P4-I | HzNV-2 detection | 5′-GCACGATTCGTAATGTTC-3′ | 51.8 * | [12] |
| P4-II | HzNV-2 detection | 5′-GCACACCTATCAATCACC-3′ | 52.8 * | [12] |
| P13-I | HzNV-2 detection | 5′-TCGATGCCGTAATACC-3′ | 49.7 * | [12] |
| P13-II | HzNV-2 detection | 5′-GTCGCTGAATCAAGTCTG-3′ | 53.2 * | [12] |
| Hz_SHMT_1738F | cSHMT TE insert intron 7 | 5′-CCAGCGCCTCTGCAAAGG-3′ | 61.4 | This Study |
| Hza_SHMT_24R | cSHMT TE insert intron 7 | 5′-TAAATGTTAAGCTGTTRTCCTCT-3′ | 53.2–55.9 | This Study |
* Tm values were recalculated for these primers using the same methods as for the PCR primers designed in this study.