Table 1.
β-galactosidase activity on unlabeled (HPTLC), AA- or pNP-labeled substrates (HPLC). All results refer to the β-galactosidases from C. gigas (NCBI Ref. Nr.: OR180112) and A. vulgaris (UniProt Ref. Nr.: A0A0B7AQJ9) [19], as both mollusk enzymes showed identical substrate specificity. ND = not determined.
| HPTLC Unlabeled Substrate |
HPLC–AA AA Labeled Substrate |
HPLC–pNP pNP Labeled Substrate |
|
|---|---|---|---|
| Galβ1,4Glc (Lactose) |
Yes (partly) | Yes | Yes |
| Galβ1,3GalNAc (Galacto-N-biose) | Yes | No | Yes |
| Galβ1,4GlcNAc (N-Acetyllactosamine) |
Yes (partly) | No | ND |
| Galβ1,6GlcNAc | No | No | ND |
| Fucα1,3[Galβ1,4]Glc (3-Fucosyllactose) |
No | No | ND |