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. 2023 Sep 29;9(10):980. doi: 10.3390/jof9100980

Figure 1.

Figure 1

Enrichment of positive phage clones specifically binding to the recombinant PbDrk1 and most abundant peptides identified. (A) Example of counting plaques on LB plates with IPTG/X-gal media with serial dilution used to phage titering; recombinant phage from the phage library that carried the lacZα gene are shown as blue plaques on the plates. (B) Quantification of blue phage plaques recovered after each round of biopanning via M13KE used in Ph.D.TM—7 Phage Display Peptide Library on immobilized PbDrk1. (C) Enrichment of phages for each selection round from the phage-displayed heptapeptide library. (D) Amino acid sequences and frequencies of occurrence from 78 phage plaques randomly selected for sequencing after three affinity-selection rounds against PbDrk1. To simplify the naming of the peptides identified, the terms Pep1 (SILPVTR), Pep2 (MPRLPPA), Pep3 (ADARYKS), and Pep6 (IPKWPTG) were adopted. Pfu: plaque-forming unit.