Figure 4.

Toxicity of selected peptides for pneumocytes A549 cells and Galleria mellonella. (A) Cytotoxicity in A549 cells determined using the resazurin method. Monolayer cells were treated with increasing concentrations of indicated peptides ranging from 25 to 1.600 µg/mL; after 24 h, metabolic activity was quantified using the resazurin assay, and the absorbance values were normalized to those of the untreated control. The result was expressed as cell viability (%) and analyzed using one-way ANOVA with Tukey’s multiple comparison test. (B) Toxicity in G. mellonella invertebrate model; groups of larvae (n = 10) were injected with 50 and 100 µg/larva of indicated peptides; survival was monitored for 7 days post-injection and analyzed using the log-rank (Mantel–Cox) test. p = 0.9447 (to Pep1), p = 0.4724 (to Pep2), p = 0.3683 (to Pep3), and p = 0.7828 (to Pep6).