Table 1.
Primers used in this work.
| Name of the Primer | Sequence (5′---3′) | Used for: | Reference |
|---|---|---|---|
| Conf-LaeA-CRISPR-FW Conf-LaeA-CRISPR-RV |
ATGTTTACGAACGGGGAT AAAGCGAGCGTTCCTGC |
Amplification of target sequence of PrlaeA gene | This work |
| RoqA-qpcr-Fw RoqA-qpcr-Rv |
ATCTGTGGCACGATTCATCA CTCGACCCTGACCATTGTTT |
rds gene expression analysis by qRT-PCR | [20] |
| RoqR-qpcr-Fw RoqR-qpcr-Rv |
TATGCCTTCAAGGGTGGTCT TTGAAGTTAGCCCAGCGAGT |
rdh gene expression analysis by qRT-PCR | [20] |
| RoqD-qpcr-Fw RoqD-qpcr-Rv |
AAAGGTTGAGGAGCACTGGA AACTCCACCCACAACTCTCG |
rpt gene expression analysis by qRT-PCR | [20] |
| adrD-qPCR-fw adrD-qPCR-rv |
GGCTCGGACGACTATACTGA AGTACAGAACGCCTGGAGTG |
adrD gene expression analysis by qRT-PCR | [12] |
| adrH-qPCR-fw adrH-qPCR-rv |
GACACCCAATATCGGACAAG AAGGCATCTGCGTGAACTAC |
adrH gene expression analysis by qRT-PCR | [12] |
| adrI-qPCR-fw adrI-qPCR-rv |
ACGTCGCGAAAAGACAAGAT TCGCGGTTGGGTAGATAAAG |
adrI gene expression analysis by qRT-PCR | [12] |
| mpaC-qPCR-FW mpaC-qPCR-RV |
CAGGGGTTCTGTGTGGGTAT AATACAGACAGCGAGCCGTA |
mpaC gene expression analysis by qRT-PCR | [7] |
| mpaG-qPCR-FW mpaG-qPCR-RV |
CGGGTAAGGGGATAGATTGT TCACATTCATAGCCACGAGA |
mpaG gene expression analysis by qRT-PCR | [7] |
| mpaH-qPCR-FW mpaH-qPCR-RV |
CCGCTGATACTACTGCCACT GCATTGAAGTTCTGCCGTAT |
mpaH gene expression analysis by qRT-PCR | [7] |
| qRT-btub-fw qRT-btub-rv |
TCCAAGGTTTCCAGATCACC GAACTCCTCACGGATCTTGG |
β-tubulin gene expression analysis by qRT-PCR | [7] |