Table 2.
Set of monoclonal antibodies (mAbs) and fluorochromes applied to analyze the expression of peripheral blood mononuclear cells (PBMCs) via surface antigen analysis.
| Specificity | Fluorochrome | Producer | Clone | Isotype |
|---|---|---|---|---|
| Mouse anti human-CD3 | FITC | BD Biosciences, USA | MEM-57 | Mouse IgG2a, κ |
| Mouse anti human-CD4 | FITC | BD Biosciences, USA | RPA-T4 | Mouse IgG1, κ |
| Mouse anti human-CD8 | FITC | BD Biosciences, USA | SK1 | Mouse BALB/c IgG1, κ |
| Mouse anti human-CD19 | FITC | BD Biosciences, USA | HIB19 | Mouse IgG1, κ |
| Mouse anti human-CD25 | PE-CyTM5 | BD Biosciences, USA | M-A251 | Mouse BALB/c IgG1, κ |
| Mouse anti human-CD69 | PE-CyTM5 | BD Biosciences, USA | FN50 | Mouse IgG1, κ |