Figure 1.
DES inhibitory activity in cells and tissues. (A) DES was tested in a dose–response assay in MDCK, A549, and Calu-3 cells. The percentages of infection were calculated by comparing the treated and untreated wells, and EC50 values were calculated with Prism 9. (B) Mucilair tissues were infected with 1 × 103 RNA copies of clinical H1N1 for 2 h at 33 °C and subsequently treated with DES. Apical washes were collected every day, and RT–qPCR was performed on them. Results are the mean and SEM of 2 to 3 independent experiments. * p < 0.0204. DES: diethylstilbestrol.