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. 2023 Oct 13;24(20):15149. doi: 10.3390/ijms242015149

Figure 5.

Figure 5

Exosomes overexpressing miR-205-5p mimics positively regulate endometrial receptivity in non-receptive endometrial epithelial cells. (a) Expression analysis of miR-205-5p in AN3-CA cells after treatment with exosomes harboring miR-205-5p mimics. (b) Analysis of ZEB1 mRNA using quantitative RT-PCR in AN3-CA cells after treatment with exosomes harboring miR-205-5p mimics. (c) Analysis of ZEB1 protein using Western blotting in AN3-CA cells after treatment with exosomes harboring miR-205-5p mimics. (d) Analysis of CDH1 mRNA using quantitative RT-PCR in AN3-CA cells after treatment with exosomes harboring miR-205-5p mimics. (e) Analysis of E-cadherin protein using Western blot analysis in AN3-CA cells after treatment with exosomes harboring miR-205-5p mimics. (f) Immunofluorescence image confirming E-cadherin expression in AN3-CA cells after treatment with exosomes harboring miR-205-5p mimics. Scale bars indicate 20 μm. (g) Spheroid attachment rate in AN3-CA cells treated with exosomes harboring miR-205-5p mimics. The data shown represent mean ± SEM from three independent experiments (n = 3). * p < 0.05.