Table 2.

Expression stability of seven candidate reference genes in S. rolfsii under different experimental conditions.

Conditions	Reference Gene	geNorm		Normfinder		Bestkeeper		ΔCt
		M	Rank	SV	Rank	CV + SD	Rank	Avg. SD	Rank
Developmental stages	18S	2.07	5	3.28	6	0.35	1	3.38	6
	28S	2.45	6	3.34	7	0.39	2	3.42	7
	EF1α	0.04	1	2.12	4	3.08	6	2.30	4
	GAPDH	0.80	3	0.29	1	2.15	4	1.84	1
	PGK	0.51	2	1.11	3	2.56	5	1.94	2
	β-TUB	0.04	1	2.17	5	3.11	7	2.34	5
	UBC	1.07	4	0.34	2	1.67	3	1.95	3
Populations	18S	0.93	4	1.02	5	0.38	1	1.30	5
	28S	1.04	5	1.35	6	0.51	4	1.48	6
	EF1α	0.51	2	0.39	2	0.77	5	1.01	2
	GAPDH	0.67	3	0.72	4	0.90	6	1.14	4
	PGK	0.16	1	0.33	1	0.40	2	0.97	1
	β-TUB	1.25	6	1.65	7	1.44	7	1.76	7
	UBC	0.16	1	0.58	3	0.44	3	1.05	3
Fungicides	18S	0.65	4	0.58	5	0.46	2	0.77	5
	28S	0.68	5	0.51	4	0.27	1	0.74	4
	EF1α	0.40	2	0.62	6	0.77	5	0.78	6
	GAPDH	0.33	1	0.34	1	0.78	6	0.64	1
	PGK	0.56	3	0.41	2	0.57	3	0.69	2
	β-TUB	0.33	1	0.49	3	0.78	7	0.69	3
	UBC	0.75	6	0.80	7	0.64	4	0.91	7
Photoperiods	18S	0.21	4	0.38	5	0.48	5	0.64	5
	28S	0.10	3	0.02	4	0.16	1	0.53	4
	EF1α	0.03	1	0.01	2	0.26	3	0.50	1
	GAPDH	0.39	5	0.97	6	0.80	6	1.01	6
	PGK	0.03	1	0.01	1	0.28	4	0.50	2
	β-TUB	0.09	2	0.02	3	0.18	2	0.51	3
	UBC	0.78	6	1.77	7	0.90	7	1.77	7
pHs	18S	0.48	6	0.61	7	0.30	4	0.67	7
	28S	0.23	3	0.20	3	0.30	3	0.41	4
	EF1α	0.11	1	0.18	2	0.38	5	0.38	2
	GAPDH	0.41	5	0.49	5	0.13	1	0.60	6
	PGK	0.17	2	0.24	4	0.39	6	0.40	3
	β-TUB	0.11	1	0.06	1	0.29	2	0.35	1
	UBC	0.30	4	0.50	6	0.48	7	0.56	5
Total	18S	1.22	5	1.21	5	0.41	2	1.46	5
	28S	1.34	6	1.52	7	0.40	1	1.65	7
	EF1α	0.55	1	0.93	4	1.38	6	1.24	3
	GAPDH	0.55	1	0.65	2	1.23	5	1.15	2
	PGK	0.62	2	0.42	1	0.99	4	1.11	1
	β-TUB	0.76	3	1.32	6	1.52	7	1.50	6
	UBC	0.99	4	0.71	3	0.73	3	1.26	4

Note: M, expression stability value; SV, stability value; CV + SD, coefficient of variation and standard deviation; Avg. SD, average standard deviation.