TLR3 activation after the treatment of HEKBlue-TLR3 and HEKBlue-null cells with necrotic fluids derived from oral squamous cell carcinoma patients: patient 1, patient 2, patient 3, patient 4 and patient 5. Ctrl—cells were treated only with fresh culture medium; RNAse—sample pretreated with RNAse; TLR3ab—sample treated with monoclonal TLR3 antibody; pIC—cells treated with 100 ng/mL poly(I:C). The amount of RNA and protein in each sample is also presented. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.001, # p < 0.05, ## p < 0.01, ### p < 0.001 (HEKBlue-TLR3 in relation to HEKBlue-null control). + p < 0.05 (HEKBlue-null patient in relation to HEKBlue-null control), $ p < 0.05 (HEKBlue-null treatment in relation to HEKBlue-null patient).