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USP17 increases osteogenic gene expression in BMP4-induced C2C12 cells. C2C12 cells were transfected with empty vector or Myc-USP17 and treated with or without BMP4 for 48 h. (a,b) The mRNA levels of ALP and OC were measured by RT-qPCR and normalized against GAPDH. The data are presented as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 compared to BMP4-treated group.
