Skip to main content
. 2023 Oct 17;24(20):15257. doi: 10.3390/ijms242015257

Figure 3.

Figure 3

Usp17 knockdown suppresses osteoblast differentiation in BMP4-induced C2C12 cells. C2C12 cells were transfected with pSuper retro puro empty vector or shUsp17 plasmid (0.25, 0.5 μg) and treated with or without BMP4 for 72 h. (a) The presentative image and quantification of ALP staining. (b) The ALP activity was measured using a commercial kit. The data are presented as the mean ± SEM. ** p < 0.01, *** p < 0.001 compared to the BMP4-treated group. (c) The protein levels of Osx, Runx2, and tubulin were measured by IB. Tubulin was used as a loading control.