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Usp17 knockdown decreases osteogenic gene expression in BMP4-induced C2C12 cells. C2C12 cells were transfected with pSuper retro puro empty vector or shUsp17 plasmid and treated with or without BMP4 for 48 h. (a,b) The mRNA levels of ALP and OC were measured by RT-qPCR and normalized against GAPDH. The data are presented as the mean ± SEM. * p < 0.05, ** p < 0.01 compared to the BMP4-treated group.
