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USP17 interacts with Osx. HEK 293 cells were transfected with HA-Osx or co-transfected with Myc-USP17. (a) The interaction between Osx and USP17 was examined by IP. (b) The interaction between purified USP17 and Osx was detected by GST pull-down assay and detected by IB. Expression of GST proteins was identified by Ponceau S staining (GST-vector: first line, GST-USP17: second line).
