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USP17 increases Osx protein expression. (a) HEK 293 cells were transfected with HA-Osx and increasing amounts of Myc-USP17 (0.125, 0.25, 0.5 μg). The protein levels were detected by IB. (b) C2C12 cells were transfected with empty vector or USP17 and treated with or without BMP4 for 48 h. The mRNA level of Osx was measured by RT-qPCR. The data are presented as the mean ± SEM. ns, not significant. ** p < 0.01 compared to the control group.
